Similar relationships exist for other experimental techniques, including light scattering, intrinsic viscosity measurement, sedimentation equilibrium, and cloud point titration.
2.
Through a series of experiments utilizing the sedimentation equilibrium technique, two important observations were made : hemoglobin has a molecular weight of 68, 000 Da, suggesting that there are four iron atoms present rather than one, and that no matter where the hemoglobin was isolated from, it had exactly the same molecular weight.
3.
He established 4 classes of proteins : water-soluble albumins; salt soluble globulins : vicilin typically having sedimentation coefficients, S values ( a measure of the protein mass determined by sedimentation equilibrium ultracentrifugation ) of about 7 Svedberg units ( hence the common name 7S globulin ) and legumin ( 11S ); alcohol / water-soluble cereal prolamines; and a fourth class, glutelins, of difficultly soluble proteins no longer recognized and now considered low solubility prolamin or globulin storage proteins.
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